One-step preparation of antibody and oligonucleotide dual-labeled gold nanoparticle bio-probes and their properties |
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Authors: | Xiao-li Kong Fei-yan Qiao Hui Qi Fu-rong Li |
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Institution: | (1) Clinical Medical Research Center, The Second Clinical Medical College (Shenzhen Peoples’ Hospital), Jinan University, 1017, North Road of Eastern Gate, Shenzhen, 518020, China |
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Abstract: | A novel method of one-step preparation of dual-labeled gold nanoparticle bio-probes was established by the electrostatic adsorption
and the covalent bonding of gold nanoparticles with antibodies and thiol-modified oligonucleotides, respectively. Characterization
of probes, the coverage and activity of antibodies and oligonucleotides on probe surfaces were detected. The results indicated
that the gold nanoparticles labeled with antibodies and oligonucleotides possess good bioactivity and the coverage of oligonucleotide
and antibody on a dual-labeled gold nanoparticle bio-probe was (92 ± 20) and (8 ± 3), respectively. The preparative method
is simple and stable. The dual-labeled gold nanoparticle bio-probes have an application value in detection of ultramicro protein. |
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Keywords: | Antibody Gold nanoparticle Nanoparticles Oligonucleotide Probe |
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