Construction of spore mutants of Bacillus subtilis for the development as a host for foreign protein production |
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Authors: | June-Hyung Kim Byung-Gee Kim |
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Institution: | (1) Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University, Seoul, 151-742, Korea |
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Abstract: | For the development of Bacillus subtilis as a host for foreign protein synthesis, three types of sigma factor deleted mutants (spoIIAC, spoIIIG and spoIIIC) were constructed by antibiotic marker insertion using plasmid vector-mediated method or LFH (Long Flanking Homology)-PCR. Mother cell specific sigma factor mutants of B. subtilis (K), B. subtilis DB104 spoIIIC (km
r)::pMK101, had two to three times higher subtilisin activity than the wild type DB104::pMK101. Subtilisin expression by the other two mutants, B. subtilis DB104 spoIIAC (km
r)::pMK101 and DB104 spoIIIG (km
r)::pMK101, which are pre-spore specific sigma factor (F and G) deleted strains, was similar to, or less than that of the wild type. |
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Keywords: | Bacillus subtilis sigma factor spore mutant subtilisin |
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