Production of inulinase by mixed culture of Aspergillus niger and Kluyveromyces marxianus |
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Authors: | Gaye Öngen-Baysal S Suha Sukan |
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Institution: | (1) Department of Food Engineering, Faculty of Engineering, Ege University, 35100 Bornova, Izmir, Turkey |
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Abstract: | Summary Inulinase activity produced by a mixed culture of Aspergillus niger and Kluyveromyces marxianus growing on Jerusalem artichoke powder was investigated. Inulinase produced by this mixed culture had a higher invertase-type activity than inulinase from respective monocultures. When hydrolysis was carried out at 50°C with Jerusalem artichoke exctract (total sugar 16% w/v) at pH 5.0, 90% hydrolysis was achieved after 4 h with 5% v/v of crude cell free enzyme preparation. |
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