Target HCV NS3 CD4+ Th1 Epitope to Major Histocompatibility Complex Class II Pathway |
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Authors: | Ming Gao Hai-Ping Wang Yan-Ning Wang Yong Zhou Quan-Li Wang |
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Institution: | (1) Lab of Blood-borne Virus, Institute of Transfusion Medicine, 27(9) Taiping Road, 100850 Beijing, China;(2) LanZhou Jincheng Hospital, 98 west street XiaoXiHu, 730050 QiLiHe, LanZhou, Gansu, China |
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Abstract: | A hepatitis C virus (HCV) plasmid vaccine was constructed, based on class II-associated invariant chain peptide (CLIP) substitution
which endogenously targets HCV non-structure protein 3 (NS3) CD4+ T helper 1(Th1) epitope (1248AA-1261AA) to major histocompatibility complex (MHC) class II antigen. The in vitro expression results demonstrated that the vaccine was expressed efficiently in COS-7 cell line. The expressed protein could
co-localize in endo-membrane system with BALB/c mouse MHC class II molecule I-Ad. The recombinant invariant chain molecule could aggregate with BALB/c mouse I-Ad molecule and form the theoretical nonomer structure in the COS-7 cell line. The assembled molecules migrate to the cell surface
by exocytosis. This has implications for HCV vaccine development. |
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Keywords: | DNA vaccine hepatitis C virus invariant chain |
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