Partial purification of nattokinase from Bacillus subtilis by expanded bed adsorption |
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| Authors: | Hong-Bo Hu Shan-Jing Yao Le-He Mei Zi-Qiang Zhu Byung-Ki Hur |
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| Institution: | (1) Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, 310027, P.R. China;(2) Department of Biological Engineering, Inha University, Inchon, 402-751, Korea |
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| Abstract: | Nattokinase was purified from unclarified Bacillus subtilisculture filtrate using an expanded bed with a purification factor of 8.2 and at a yield of 95%. The optimal pHs for adsorption and elution were 6.0 and 7.0, respectively. The expanded bed route shortened the process time by 8–10 h and increased the yield by 50% when compared with the conventional method. |
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| Keywords: | adsorption expanded bed nattokinase thrombolytic activity |
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