Cryopreservation of hairy root cultures of Vinca minor (L.) by encapsulation-dehydration |
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Authors: | Kazumasa Hirata Misuzu Mukai Saeko Goda Masumi Ishio-Kinugasa Kenji Yoshida Akira Sakai Kazuhisa Miyamoto |
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Institution: | (1) 1-6 Yamada-oka, Suita Osaka, 565-0871;(2) Asabu-cho 1-5-23, Kita-ku Sapporo, 001, Japan |
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Abstract: | Hairy root cultures of Vinca minor and Ajuga reptans var. atropurpurea could be cryopreserved when the roots were precultured and encapsulated in 2% (w/v) alginate beads with 0.3 M sucrose and 0.5 M glycerol and dehydrated until the bead weight reached 25% of the initial weight before cooling in liquid nitrogen. Preculture and encapsulation of the roots with abscisic acid was effective in increasing the survival rates. For V. minor root tips moreover a sufficiently high survival rate of more than 70% was attained by eliminating glycerol from the preculture medium and dehydration of beads until 23% of the initial weight was reached instead of 25%. |
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Keywords: | abscisic acid cryopreservation encapsulation-dehydration hairy root culture Vinca minor |
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