Supplement of nutrients for effective cultivation of hepatitis B surface antigen-producing recombinant yeast |
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| Authors: | Kyung Hwan Jung Mahn Hoon Park Hong Mo Moon Joon Shick Rhee |
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| Institution: | (1) Department of Biotechnology, Korea Advanced Institute of Science and Technology, 373-1, Kusong-Dong, Yusong-Ku, 305-701 Taejon, Korea;(2) Mogam Biotechnology Research Institute, 341 Pojung-Ri, Koosung-Myon, Yongin-Kun, 449-910 Kyonggi-Do, Korea |
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| Abstract: | Summary In the hepatitis B surface antigen (HBsAg)-producing recombinant yeast culture medium, the supply of Bacto-yeast nitrogen base without amino acids was found to be inadequate due to the lack of the several kinds of vitamins and trace elements. When the culture medium for this recombinant yeast was supplemented with sufficient vitamins and trace elements, its growth, HBsAg production and the stability of plasmid were improved. |
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