Locally administered lentivirus-mediated siRNA inhibits wear debris-induced inflammation in murine air pouch model |
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| Authors: | Xiaochun Peng Xianlong Zhang Bingfang Zeng |
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| Institution: | (1) Department of Orthopaedics, The Sixth Affiliated People’s Hospital, Shanghai Jiaotong University, Shanghai, 200233, China |
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| Abstract: | Lentivirus(LV)-mediated small interfering RNA (siRNA) targeting tumor necrosis factor-α (TNF-α) was locally administered into
the air pouch of mice to inhibit inflammation induced by titanium alloy particles. The lentiviral vector expressed green fluorescent
protein (GFP) as a reporter gene. Down-regulation of TNF-α in pouch area was confirmed by real-time PCR and ELISA, resulting
in significantly decreased local inflammatory responses (P < 0.01). This approach was proven safe by localized GFP fluorescence and invariant TNF-α expression in peripheral blood,
liver, spleen, kidney, lung and brain of mouse. In conclusion, locally administered siRNA provides an effective and safe method
for inhibiting particle-induced inflammation. |
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| Keywords: | Inflammation Lentiviral vector Local administration Small interfering RNA Tumor necrosis factor-α |
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