Application of a Fab fragment of monoclonal antibody specific to <Emphasis Type="Italic">N</Emphasis>-terminal pro-brain natriuretic peptide for the detection based on regeneration-free electrochemical immunosensor |
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Authors: | Weijing Yi Wenbin Liang Peng Li Shuhui Li Zhujun Zhang Mingzhen Yang An Chen Bo Zhang Chuanmin Hu |
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Institution: | (1) Department of Clinical Biochemistry, Laboratory Sciences, Third Military Medical University, Chongqing, 400038, People’s Republic of China;(2) Department of Cardiology, The Second Affiliated Hospital, Third Military Medical University, Xinqiao Street, Shapingba District, Chongqing, 400037, People’s Republic of China; |
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Abstract: | N-Terminal pro-brain natriuretic peptide (NT-proBNP) is an important marker for heart failure that reflects ventricular volume
expansion, ventricular overload and the degree of cardiac injury. To establish a sensitive and simple detection method for
it in serum, a regeneration-free immunosensor with novel Fab fragment monoclonal antibodies was prepared. The sensor detected
NT-proBNP from 0.04 to 2.5 ng/ml with a limit of 0.03 ng/ml. The immunosensor is therefore a simple, cost-effective method
to detect NT-proBNP and the proposed immunoassay system would enable other proteins to be detected and open new opportunities
for protein diagnostics. |
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Keywords: | |
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