Purification of coagulation factor VIII using chromatographic methods. Direct chromatography of plasma in anion exchange resins |
| |
Authors: | Elisabeth Cheng Daniela Jinzenji Ana Paula Almeida Aranha Lorthiois Roberta Rodrigues de Carvalho Anita Mitiko Tanaka-Azevedo Isaías Raw Elizabeth Angélica Leme Martins |
| |
Institution: | 1.Centro de Biotecnologia,Instituto Butantan,S?o Paulo,Brazil;2.Programa de Pós-gradua??o Interunidades em Biotecnologia,Universidade de S?o Paulo,S?o Paulo,Brazil |
| |
Abstract: | Coagulation factor VIII (FVIII) concentrates are used in the treatment of patients with Hemophilia A. Human FVIII was purified
directly from plasma using anion exchange chromatography followed by gel filtration. Three Q-Sepharose resins were tested,
resulting in 40% recovery of FVIII activity using Q-Sepharose XL resin, about 80% using Q-Sepharose Fast Flow and 70% using
the Q-Sepharose Big Beads. The vitamin K-dependent coagulation factors co-eluted with FVIII from the anion exchange columns.
In the second step of purification, when Sepharose 6FF was used, 70% of FVIII activity was recovered free from vitamin K-dependent
factors. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|