Production of acidic xylo-oligosaccharides by a family 10 endoxylanase from Thermoascus aurantiacus and use as plant growth regulators |
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Authors: | P Katapodis A Kavarnou S Kintzios E Pistola D Kekos BJ Macris P Christakopoulos |
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Institution: | (1) Biotechnology Laboratory, Department of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens, 157 80, Greece;(2) Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece |
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Abstract: | Neutral and acidic oligosaccharides were obtained from birchwood xylan by treatment with an endoxylanase, family 10 class, from Thermoascus aurantiacus. The main acidic xylooligosaccharide (aldotetrauronic acid) was separated from the hydrolysate by anion-exchange and size-exclusion chromatography and the primary structure was determined by 13C NMR spectroscopy. The aldotetrauronic yield was 15% (w/w) of the total solubilised sugars. The addition of purified aldoterauronic acid at 1.6–16 mg l–1 growth medium, induced callus and somatic embryogenesis in culture explants of common mallow (Malva silvestris L.) and cotton (Gosssypium hirsutum). |
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Keywords: | acidic oligosaccharides plant growth regulators xylanase |
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