Sequential immobilization of urease to glycidyl methacrylate grafted sodium alginate |
| |
| Institution: | 1. Polymer Laboratory, Instituto de Ciencia y Tecnología de Materiales, Universidad de la Habana, Zapata y G, Vedado, La Habana, Cuba;2. Department of Materials Science and Engineering, Pontifícia Universidade Católica do Rio de Janeiro, R. Marquês de São Vicente, 225 – Gávea, Rio de Janeiro, RJ 22451-000, Brazil;3. Physico-Chemical Department, Facultad de Química, Universidad de la Habana, San Lázaro y L., Plaza de la Revolución, La Habana, Cuba;4. Physics Department, Universidade Federal do Amapá, Rod. Juscelino Kubitschek, km-02 Jardim Marco Zero, Macapá, AP CEP 68.903-419, Brazil;1. Chemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt;2. Medical Laboratory Technology Department, Faculty of Allied Medical Sciences, Pharos University, Alexandria, Egypt;3. Chemistry Department, Faculty of Science, Damanhour University, Damanhour, Egypt;1. School of Life Science, Liaoning Normal University, Dalian 116081, China;2. Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China;3. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;1. Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China;2. Institute of Environment Remediation and Human Health, And College of Ecology and Environment, Southwest Forestry University, Kunming, 650224, China;3. State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China;4. Institute of Bio- and Geosciences, Agrosphere (IBG-3), Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Straße, 52425, Jülich, Germany |
| |
| Abstract: | Objective of this study is to realize appropriate enzyme immobilization onto a suitable support material and to develop a model which enables reactions catalyzed with different enzymes arranged in order. Thence, this model was potential for developing a multi-enzyme system. The reactions need more than one enzyme can be realized using immobilized form of them and the enzymes will be in one support at wanted activities. In this study, sodium alginate was used as immobilization material and glycidyl methacrylate was grafted onto sodium alginate. Thus reactive epoxy groups were added to sodium alginate which also has carboxyl groups. Average molecular weight of sodium alginate was determined using Ubbelohde viscosimetri. The molecular mass of sodium alginate was calculated as 15,900 Da. Graft polymerization was made in two steps. Firstly, sodium alginate was activated with benzophenone using UV-light at 254 nm. Secondly, glycidyl methacrylate was grafted under UV-light at 365 nm onto activated sodium alginate. Grafted glycidyl methacrylate was determined gravimetric and titrimetric. Additional groups after grafting were showed with FT-IR spectrum. 1-Ethyl-3-(3-dimetylaminopropyl)-carbodiimide was used for immobilization urease from carboxyl groups at pH 5.0. Suitable 1-ethyl-3-(3-dimetylaminopropyl)-carbodiimide/–COOH ratio was found 1/10 and immobilized product activity was 197 U/g support. Reaction medium pH was 8.0 for immobilization from epoxy group. Optimum immobilization reaction time was found as 2 h and immobilized product activity was 285 U/g support. Sequential immobilization of urease to glycidyl methacrylate grafted sodium alginate was made from –COOH and epoxy groups, respectively. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
