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Enantioselective epoxide hydrolase activity of a newly isolated microorganism, Sphingomonas echinoides EH-983, from seawater |
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| Authors: | Hee Sook Kim Ok Kyung Lee Soo Jung Lee Seungha Hwang Sang Jin Kim Sung-Hyun Yang Sunghoon Park Eun Yeol Lee |
| Institution: | aDepartment of Food Science and Technology, Kyungsung University, Busan 608-736, Republic of Korea bSchool of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea cMarine Biotechnology Research Centre, Korea Ocean Research & Development Institute, Ansan 426-755, Republic of Korea dDepartment of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea |
| Abstract: | A marine microorganism, Sphingomonas echinoides EH-983, which possesses epoxide hydrolase (EH) activity was isolated from seawater and characterized. The EH of S. echinoides EH-983 preferentially metabolized (R)-enantiomer when the racemic styrene oxides were supplied as substrates. The optimal pH and temperature for the enantioselective hydrolysis by whole-cells ofS. echinoides EH-983 were 7.0 and 20 °C, respectively. When kinetic resolution was conducted with a racemic mixture of styrene oxides at an initial concentration of 40 mM, enantiopure (S)-styrene oxide was obtained in 180 min with a yield of 21.3%. To our best knowledge, S. echinoides EH-983 is the first marine microorganism that is reported to have EH activity. |
| Keywords: | Enantioselective hydrolysis Epoxide hydrolase Sphingomonas echinoides EH-983 Marine microorganism |
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