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A novel transfection method for eukaryotic cells using polyethylenimine coated albumin microbubbles |
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| Authors: | Dang Shi-peng Wang Ru-xing Qin Ming-de Zhang Yan Gu Yan-zheng Wang Ming-yuan Yang Qiao-lin Li Xiao-rong Zhang Xue-guang |
| Institution: | a Institute of Medical Biotechnology, School of Medicine, Soochow University, Suzhou 215007, China b Department of Cardiology, Affiliated Hospital of Nanjing Medical University in Wuxi, Wuxi Peoples’ Hospital, Wuxi 214023, China c Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou 215123, China d The Red Cross Blood Center of Suzhou, Suzhou 215006, China e Department of Parasitology, School of Medicine, Soochow University, Suzhou 215123, China |
| Abstract: | Albumin microbubbles have been intensively studied for their application in gene delivery. However, with negative surface potential, albumin microbubbles hardly bind plasmid DNA, which might contribute to their low transgene efficiency. In this study, we developed polyethylenimine (PEI) coated albumin microbubbles (PAMB) which were prepared by sonicating the mixture of human albumin, PEI, polyethylene glycol and glucose. CHO cells, COS cells and 293T cells were transfected with PEI, PEI + albumin, PAMB and Lipofectamine 2000, respectively. Our results showed that the surface potential was elevated and PAMB could bind plasmid DNA. The transgene efficiency of PAMB was higher than PEI and PEI + albumin (P < 0.05), and PAMB performed the same transgene effect as Lipofectamine 2000 did but with lower cytotoxicity than Lipofectamine 2000. Albumin microbubbles modified by PEI has high transgene efficiency and low cytotoxicity even without ultrasound medication, making it a useful non-virus gene delivery method in vitro. |
| Keywords: | Polyethylenimine Albumin microbubbles Gene delivery Transfection Ultrasound |
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