Production and characterisation of exopolysaccharide from Streptomyces carpaticus isolated from marine sediments in Egypt and its effect on breast and colon cell lines |
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| Authors: | Manal S Selim Shaimaa K Amer Sahar S Mohamed Marwa M Mounier Hala M Rifaat |
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| Institution: | 1. Microbial Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Egypt;2. Microbiology Department, Faculty of Science, Ain Shams University, Egypt;3. Pharmacognosy Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Egypt;4. Microbial Chemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre, Egypt |
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| Abstract: | Twenty streptomycete strains were isolated from marine sediment samples collected from Nabq area, Sharm El-Sheikh, Red Sea Coast, Egypt. Four of them produce exopolysaccharides (EPS) showing marked in vitro antitumor activities. Morphological and cultural characteristics of the most significant strain (No. 3) were shown. Moreover, the sequence of this strain showed similarity with Streptomyces carpaticus. The results reveal that EPS produced by Streptomyces carpaticus No. 3 had high cytotoxicity reaching 51.7% and 59.1% against human tumor cells of breast and colon lines respectively. A chemical analysis of EPS indicated that the composing monosaccharides were galactouronic acid, glucose, xylose, galactose, mannose, and fructose with relative ratio of 3:1:1:2:2:1 respectively, with an average molecular weight (Mw) 1.180 × 105?g/mol and of a number average molecular weight (Mn) 1.052 × 105?g/mol. Also the EPS contained uronic acid (0.5072%) and monosaccharide sulphates (21.753%). |
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| Keywords: | Marine streptomycetes Exopolysaccharide Antitumor Radical scavenging activity |
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