短柄枹种群遗传多样性的ISSR分析

利用ISSR分子标记方法对分布在浙江省境内的7个短柄枹种群的遗传多样性和遗传分化进行了分析。从100个引物中筛选出12个用于正式扩增的ISSR引物，在7个种群140个个体中共检测到132个位点，其中多态位点118个，多态位点百分率(P)为89.39%，各种群P平均为58.87%。短柄枹总的Shannon信息指数(I)为0.493 3、Nei指数(h)为0.334 7，各种群I平均为0.336 2、h平均为0.229 1。P、I、h均显示云峰种群最高，天台山种群最低。AMOVA分子差异分析表明，67.97%的变异存在于种群内，32.03%的变异存在于种群间，种群间的基因分化系数(G_ST)为0.315 4。短柄枹种群间的基因流为(N_m)为1.085 3。7个种群的平均遗传距离为0.173 9。利用UPGMA法对7个种群进行聚类，结果显示天台山和雪窦山种群聚成一类，其它5个种群聚成另一类。

Genetic Diversity of Quercus glandulifera var. brevipetiolata Populations Revealed by ISSR Markers

Using inter-simple sequence repeat(ISSR) makers,genetic diversity and genetic differentiation of Quercus glandulifera var.brevipetiolata populations,which distribute in Zhejiang Province,were analyzed.A total of 132 bands were amplified from 7 populations with 140 individulas by 12 reliable primers,which was selected from 100 primers.Of them,118 bands were polymorphic and the percentage of polymorphic loci of Quercus glandulifera var.brevipetiolata species was 89.39%.The percentage of polymorphic loci of Quercus glandulifera var.brevipetiolata populations averaged 58.87%.Shannon information index(I) and Nei's gene index(h) at species level were 0.493 3 and 0.334 7,respectively,while those at population level averged 0.336 2 and 0.229 1,respectively.It suggested that the genetic diversity of YF population was the highest while that of TTS population was the lowest.Analysis of molecular various showed that 67.97% of the total variation was distributed among populations,while only 32.03% was distributed among individuals within populations.The gene differentiation coefficient(GST) among populations is 0.315 4.The gene flow among populations is 1.085 3.The average genetic distance of 7 populations was 0.173 9.Using unweighted pair group method arithmatic average(UPGMA),TTS population and XDS population were clustered into one group and the other five populations were clustered into another group.