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| 漂白前后塔拉纤维剩余物的酶动力学分析 | |
| 引用本文： | 路祺,彪林海,徐蕾,孟永斌,祖元刚,聂思铭,孟庆涣.漂白前后塔拉纤维剩余物的酶动力学分析[J].植物研究,2015,35(5):791-794. |
| 作者姓名： | 路祺 彪林海 徐蕾 孟永斌 祖元刚 聂思铭 孟庆涣 |
| 作者单位： | 1.生物资源生态利用国家地方联合工程实验室，哈尔滨 150040； 2.东北林业大学森林植物生态学教育部重点实验室，哈尔滨 150040 |
| 摘 要： | 对塔拉纤维剩余物和漂白塔拉纤维剩余物的酶动力学进行了研究，采用Lineweaver-Burk双倒数法对底物样品浓度倒数和酶促反应速度倒数作图构建米氏方程，通过计算两种底物的Km值，即塔拉纤维剩余物Km为12.73 mg·mL-1，漂白塔拉纤维剩余物Km为192 mg·mL-1，表明塔拉纤维剩余物比漂白后的塔拉纤维剩余物更适合做纤维素酶的酶解底物；红外光谱图分析表明漂白塔拉纤维剩余物中木质素成分含量明显降低。 |
| 关 键 词： | 塔拉 纤维剩余物 漂白 酶动力学 米氏方程 |
Enzyme Kinetics of Tara Fiber Residues Before and after Bleaching |
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| LU Qi,BIAO Lin-Hai,XU Lei,MENG Yong-Bin,ZU Yuan-Gang,NIE Si-Ming,MENG Qing-Huan.Enzyme Kinetics of Tara Fiber Residues Before and after Bleaching[J].Bulletin of Botanical Research,2015,35(5):791-794. | |
| Authors: | LU Qi BIAO Lin-Hai XU Lei MENG Yong-Bin ZU Yuan-Gang NIE Si-Ming MENG Qing-Huan |
| Institution: | 1.National Local Joint Engineering Laboratory for Ecological Utilization of Biological Resources,Harbin 150040； 2.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040 |
| Abstract: | We studied the enzyme kinetics of Tara fiber residues before and after bleaching. By the Lineweaver-Burk double reciprocal plot method, we used the reciprocals of sample concentration and enzymatic reaction rate to plot and construct Michaelis equation. Km value of two substrates were 12.73 mg·mL-1 and 192 mg·mL-1 by calculating, respectively, and the Tara fiber residues was more suitable than the bleached Tara fiber residues. The sample of bleached Tara fiber residues had lower lignin content by FTIR. |
| Keywords: | Tara fiber residues bleaching enzyme kinetics Michaelis equation |
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