黑龙江省蹄盖蕨科三属(蹄盖蕨属、羽节蕨属及冷蕨属)的遗传多样性分析

利用7种随机引物对分布于黑龙江省不同区域蹄盖蕨科中的蹄盖蕨属,羽节蕨属及冷蕨属三属的遗传多样性进行了初步分析,对蹄盖蕨科各种基因组的DNA进行了PCR扩增,并对其RAPD谱带进行了统计处理,得出不同区域种类的多态位点百分率,结果表明蹄盖蕨科植物具有较高水平的遗传变异,其种类的分布对周围环境变化有着较强的适应能力.利用Shannon指数和Nei指数对其分析,确定了三属之间的属内种间的亲缘关系:蹄盖蕨属的大部分变异仍存在于种源内;冷蕨属中的冷蕨和山冷蕨种类之间具有一定的遗传变异;羽节蕨属中,欧洲羽节蕨和羽节蕨种间有较大的遗传变异.

RAPD analysis of the genetic diversity in Athyrium, Gymnocarpium and Cystopteris of Athyriaceae from Heilongjiang Province

random primers have been used in studying the genetic diversity of Athyriaceae from different region in Heilongjiang Province. The genomic DNA of Athyriaceae have been amplified by PCR. Then the RAPD bands have been statistically analyzed and the percentage of polymorphism were obtained. The data suggest Athyriaceae plants have high level genetic variations and the distribution of its specimens have well adaptive capacity to environment changes. The data have also been further analyzed by Shannon and Nei index to estimate the relationship of specimens within and among the three genus:Athyrium, Gymnocarpium and Cystopteris. The results show: Most variations of Athyrium are still exist among its specimens; There is some variations between Cystopteris fragilis and Cystopteris sudetica in Cystopteris to some degree; In Gymnocarpium, the genetic variations between Gymnocarpium dryopteris and Gymnocarpium jessoense are somewhat prominent. The results establish a foundation in the study of taxonomic status of Athyrium, Gymnocarpium and Cystopteris in Athyriaceae.