84K杨愈伤组织再生体系和直接分化再生体系遗传转化的比较性研究

植物转基因必须以建立良好的植物转化受体系统为基础，而良好的受体系统必须以具有高频率的转化率及较强的再生能力为前提。实验以84K杨叶片为材料研究其在不同培养条件、不同种类及浓度的植物激素对其脱分化及再分化的影响，并建立了两种成熟的组织再生系统，得出组织培养体系与遗传转化体系的受体系统在激素配比方面存在一定差距，所以在此基础上进行了PCK1、PCK2两个基因的愈伤途径和不定芽途径途径的遗传转化，并对两种遗传转化体系进行了对比性分析并总结出两种体系的优缺点。

The Comparative study of Callus and Direct Differation Regenaration System of 84K Poplar

High-frequent transformation and strong regeneration ability of plants are prerequisites to the establishment of good plant transformation receptor system, which is the foundation for plant genetic transformation. Taking 84K poplar leaves as study material, this experiment studies the influence of different types and concentrations of plant hormones to its dedifferentiation and re-differentiation under different cultivation conditions, and sets two mature plant tissue cultivation regeneration system. Based on above,here establishes genetic transformation system of adventitious buds and callus pathway, respectively as callus regeneration system and regeneration system of direct differentiation. Then through conducting comparative analysis of two tissue culture system using PCK1 and PCK2, here summarizes the advantages and disadvantages of two methods.