不同pH值与电位耦合对牛血清白蛋白包被酒石酸长春瑞滨纳米粒制备工艺的影响

在反溶剂法制备纳米粒过程中,pH值在一定程度上会对其产生影响。本文通过在不同酸碱环境下运用反溶剂法制备牛血清白蛋白包被酒石酸长春瑞滨纳米粒,进而借助于电位耦合作用来研究纳米粒制备工艺。研究结果表明:当pH=4.5至7.5时,酒石酸长春瑞滨和牛血清白蛋白带有异种电荷,而当pH=2.5,3.5,8.5,9.5时它们均带有同种电荷。当pH=7.5时,牛血清白蛋白带有负电荷即-8.52 mV,酒石酸长春瑞滨带有正电荷即+4.48mV。此时得到牛血清白蛋白包被酒石酸长春瑞滨纳米粒粒径为193.3 nm,Zeta电位为-30.86 mV,而且在该pH下对纳米粒制备工艺进行了优化,最终它的载药量和包封率达到了45.6%和90.6%。

Effects of pH Value and Potential Coupling on the Preparation of Vinorelbine Tartrate Loaded Bovine Serum Albumin Nanoparticles

pH value plays an important role in preparing nanoparticles using antisolvent procedure.In this research,vinorelbine tartrate loaded bovine serum albumin nanoparticles were prepared at different pH values.The preparation process of nanoparticles was analyzed in the view of potential coupling.The results indicated that vinorelbine tartrate and bovine serum albumin took the opposite charges at pH value from 4.5-7.5.However,they took the same charge at pH 2.5,3.5,8.5 and 9.5.Especially,vinorelbine tartrate and bovine serum albumin took +4.48 and-8.52mV respectively.The particle size of vinorelbine tartrate loaded bovine serum albumin nanoparticles reached 193.3nm and zeta potential reached-30.86mV in this condition.Moreover,the preparation process of nanoparticles were optimized at this pH value and the drug loading and encapsulation efficiency of this drug were approximately up to 45.6% and 90.6%,respectively.