叶酸白蛋白靶向纳米粒的肿瘤细胞吸收及叶酸受体相关基因表达研究

实验选用叶酸受体表达阳性FR(+)卵巢癌细胞株SKOV3与表达阴性的FR(-)肺癌细胞株A549,通过肿瘤细胞对叶酸白蛋白靶向纳米粒的吸收及叶酸受体相关基因的表达,研究分析叶酸白蛋白靶向纳米粒叶酸受体吸收特征及叶酸受体胞吞途径的参与机制。结果表明:随着叶酸白蛋白靶向纳米粒给药时间的增加,SKOV3细胞FR1基因的表达显著增强,而A549细胞未见FR1基因表达。同时SKOV3细胞FR1基因的表达程度与细胞摄取叶酸白蛋白靶向纳米粒的量成正相关。叶酸代谢通路相关的基因(RFC、FPGS、GGH)表达,研究显示叶酸白蛋白靶向纳米粒能够启动细胞膜叶酸通道相关蛋白的表达。

Tumor Cell Absorption of Folate Conjugated Albumin Nanoparticle and Folate Receptor-related Gene Expression

To investigate the effect of folate conjugated albumin nanoparticle on tumoral cell,the folate receptor positive FR(+) SK-OV-3 cell and FR(-) lung A549 cell were used for experiment.By the techniques of cell culture in vitro and semi QRT PCR,SKOV3 cell and A549 cell were treated by folate conjugated albumin nanoparticles in various concentrations for 24 h.Folate conjugated albumin nanoparticles significantly increased the expression of FR1 gene of SKOV3 cell.There was a positive correlation between the concentration of folate conjugated albumin nanoparticle and the expression of FR1 gene.Folate conjugated albumin nanoparticle increased the activity of FOLR1 protein and related gene,which may be one of important endocytosis mechanisms of folate conjugated albumin nanoparticle.