植物叶片愈伤组织形成的可能机制

分析了植物叶片在组培条件下形成愈伤组织的过程.文中提出,培养基配方中的酸性物质使植物叶片处于酸性环境中并导致植物正常细胞首先发生细胞壁酸性降解,随后出现原生质体脱离细胞壁,进一步发生细胞器重组或细胞重建,人工培养基的酸性环境使细胞壁强制性地降解后,植物原生质体失去细胞壁的包被后直接处于较酸性的环境中,可能会促使原生体出现酸性快速分裂.因此,植物细胞壁是控制植物细胞完成正常细胞周期的信号载体.

Possible mechanisms involved with the formation of leaf callus

This paper attempted to illustrate the mechanisms of callus formation of plant leaf. The theory was proposed that the acid material in the culture medium maintained the plant leaf in the acid environment, and this led to fast degradation of cell wall, breaking away of protoplast from the cell wall. These free protoplasts will aggregation into callus, without fixed organization and functions and being ready to recombine or rebuild for the further differentiation. The acid condition in the culture medium played the most important role in the dedifferentiation of leaves and in the formation of callus, during this course plant cell wall was presumed to serve as signal carrier controlling the cell to finish the normal cell's cycle, not only as the inactive coat of cells.