用显微分离和超敏感银染电泳方法研究百合减数第一分裂周期蛋白质变化

利用"显微操作人工分离单细胞"、"微量蛋白SDS-PAGE"和"超敏感银染"技术对百合花粉母细胞减数第一分裂周期蛋白质组成与变化作了比较研究。100个百合减数分裂单细胞可检测到25条以上的蛋白质谱带。前期与中期蛋白质谱带数与谱型基本相似,并且未呈现明显变化。后期和末期谱型相同,但与前期和中期相比出现明显变化, 185KD谱带在后期和末期中出现, 28KD、27KD、26KD、24KD、23KD、22KD、18KD、16KD 8条谱带消失和H4变浅, H3完全消失。可以认为,本工作建立的实验方法和所得初步结果对开展高等植物减数分裂周期调控生化机理研究有一定参考价值。

STUDIES ON PROTEIN CHANGES OF POLLEN MONTHER CELLS DURING MEIOSIS BY MICRO-ISOLATION AND ULTRASENSITIVE SILVER STAIN TECHNIQUES IN LILIUM DAVILL DUCH

In this paper, the comparative analysis of protein composition and change in pollen monther cells have been studied during meiosis I cycle by using "Micro-Isolation"、"Micro-Protein SDS-PAGE"and "Ultrasensitive Silver Stain" techniques. The results indicated that more than 25 protein bands could be detected in 100 meiotic cells which were isolated by micromanipulation. Protein composition has not change at prophase I and metaphase I. But 8 bands which have molecular weight 28KD、27KD、26KD、26KD、24KD、23KD、22KD、16KD、, respectively, were disappeared and 185KD protein band was appeared at anaphase I and telophase I. Experiment techniques were set up in this study are very useful for studying protein changes during meiosis and experiment results were obtained in this study are also very valuable for studying biochemical mechanism of meiotic cycle and cycle regulation in higher plants.