凉水国家自然保护区天然红松种群遗传多样性在时间尺度上变化的cpSSR分析

应用叶绿体微卫星（cpSSR）技术对凉水国家自然保护区天然红松种群的遗传多样性在时间尺度上的变化进行了遗传分析。共选择121个树龄在1 581~1 900年间的红松个体，连续分为6个龄级。从6对叶绿体SSR引物筛选出两对引物，共检测到7个位点，其中多态位点5个，多态位点比率为71.43%。利用POPGENE分析软件计算了红松各龄级内的遗传多样性指数，分析了遗传多样性在红松各龄级间的变化。研究结果表明，凉水保护区内的红松遗传多样性在这320年间没有发生太大的波动，龄级间遗传多样性分化不明显，红松遗传多样性主要存在于龄级内。

cpSSR Analysis of Variation of Genetic Diversity in Temporal Dimension of Natural Population of Pinus koraiensis in Liangshui National Nature Reserve

With cpSSR method, the variation of genetic diversity in temporal dimension of natural population of Pinus koraiensis in Liangshui national nature reserve was studied in this paper. Total 121 P. koraiensis samples aged from 101 to 420 years were grouped into 6 age classes. 5 polymorphic loci were obtained from 2 pairs of SSR primers and the percentage of SSR polymorphic loci was 71.43%.With the POPGENE analysis software, the genetic diversity index was calculated and the variation of genetic diversity was analyzed in every age class level. The results showed that the genetic diversity of P. koraiensis in Liangshui National Nature Reserve didn’t fluctuate very much during 320 years. The differentiation of genetic diversity is not obvious in age class level, and genetic diversity of P. koraiensis primarily existed within age class.