甘草根茎乙醇提取物抗菌活性研究

本实验采用琼脂扩散法和微量肉汤稀释法，研究了甘草根茎乙醇提取物对5种细菌(表皮葡萄球菌、金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和绿脓杆菌)和2种真菌(白色念珠菌和黑曲霉)的抗菌活性。结果表明，甘草根茎乙醇提取物对革兰氏阳性菌非常敏感，而对革兰氏阴性菌和真菌不敏感，80%乙醇提取物对革兰氏阳性菌的MIC范围为0.156～0.312 mg·mL^-1，而10%乙醇提取物对革兰氏阳性菌的MIC范围为0.625～1.250 mg·mL^-1,表明甘草根茎抗菌活性成分在高浓度乙醇中溶解度较大，为临床上应用甘草根茎醇提物作为抗菌制剂提供了科学依据。

The antimicrobial activity of ethanolic extracts from the licorice (Glycyrrhiza uralensis Fisch.)roots

The ethanolic extracts from licorice (Glycyrrhiza uralensis Fisch.) roots were examined for antimicrobial activity against five bacteria (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa) and two fungi (Candida albicans, Aspergillus nigar) by using disc diffusion method and broth micro dilution method. Results showed, ethanolic extracts from the licorice roots exhibited high susceptivity against gram-positive bacteria, while no sensitivity against gram-negative bacteria and fungi. The range of MIC of 10% ethanolic extract against gram-positive bacteria is 0.156~0.312 mg·mL^-1, while that of 80% ethanolic extract is 0.625~1.250 mg·mL^-1.The results indicated that antibacterially active components of licorice roots can dissolved in higher concentration ethanol more easily, which provides scientific basis for the application of ethanolic extracts from the licorice roots as antibacterial preparation in clinic.