高效液相色谱法同时测定楠竹叶提取物中的4种黄酮

采用高效液相色谱法同时测定楠竹叶提取物中的4种主要黄酮成分：荭草苷、牡荆苷、牡荆苷-4′-O-葡萄糖苷、牡荆苷-2′-O-鼠李糖苷。通过实验，得到了同时测定4种主要黄酮成分的最佳条件。采用Kromasil-C18(5 μm，4.6 mm×250 mm，Akzol Nobel，Sweden)进行测定，最佳检测条件为：以四氢呋喃∶乙腈∶甲醇∶0.5%醋酸水溶液=10.3∶1∶0.7∶88为色谱流动相等度洗脱；检测波长360 nm；柱温25℃，流速：1.0 mL·min^-1。荭草苷、牡荆苷、牡荆苷-4′-O-葡萄糖苷和牡荆苷-2′-O-鼠李糖苷的平均回收率分别为98.5%、98.1%、99.1%和98.7%，相对标准偏差分别为0.8%、0.7%、0.8%和0.6%(n=5)。该方法分离效率高、分析速度快、检测灵敏度高、重复性好，可用于楠竹叶成分的检测分析。

Simultaneous Determination of 4 Flavonoids in Extracts of Phyllostachys pubescens Leaves by HPLC

In this study, 4 flavonoids in Phyllostachys pubescens extracts i.e. orientin, vitexin, vitexin-4′-O-glucoside, and vitexin-2′-O-rhamnose glycoside were simultaneously detected. The HPLC detected conditions were as follows: Kromasil-C18 column (5 μm, 4.6 mm×250 mm, Akzol Nobel, Sweden), mobile phase was tetrahydrofuran∶acetonitrile∶methanol∶0.5% aqueous acetic acid (10.3∶1∶0.7∶88), isocratic elution, the detection wavelength was 360 nm, column temperature was 25℃, and 1.0 mL·min^-1 flow velocity. The average recoveries of orientin, vitexin, vitexin-4′-O-glucoside, and vitexin-2′-O-rhamnose glycoside were 98.5%, 98.1%, 99.1%, and 98.7%, respectively. The relative standard deviations were 0.8%, 0.7%, 0.8% and 0.6%(n=5), respectively. This HPLC method has been proved to be a method with high efficiency, rapid analysis, high sensitivity and good reproducibility for detection of flavonoids in P.pubescens extracts.