灯盏花花药培养初报

对灯盏花花药培养诱导单倍体植株进行了研究。结果显示：灯盏花花药愈伤组织培养以附加60 g·L^-1蔗糖较好，B5和MS培养基相比较，MS培养基较适宜，在MS+NAA 1.0 mg·L^-1+BA 0.5 mg·L^-1+蔗糖60 g·L^-1的培养基中，花药愈伤组织诱导率可达36.03%。将愈伤组织转移到MS+6-BA 1.0 mg·L^-1中继代增殖后，经芽苗分化、生根后可得到完整植株。再生植株根尖细胞经细胞学鉴定存在单倍体。

Preliminary Study on Anther Culture of Erigeron breviscapus

The preliminary study was carried out on the anther culture of Erigeron breviscapus. The results showed that during callus induction, 60 g·L^-1 sucrose was suitable；MS medium was better than B5; the highest induction efficiency 36.03% was achieved on the medium of MS+NAA 1.0 mg·L^-1+BA 0.5 mg·L^-1+sucrose 60 g·L^-1. calli were transferred on medium MS+6-BA 1.0 mg·L^-1 to proliferation. After differentiating and taking roots, plantlets were obtained. The chromosome numbers from root-tip cells of regeneration plant was nine. It showed that the regeneration plant was haploid.