酸性培养基对喜树叶片细胞壁降解的影响

在组织培养过程中对喜树叶片外植体进行解剖学观察。发现在组织培养条件下,喜树叶片在培养基的酸性环境中细胞壁呈现模糊微弱降解、明显降解和完全降解直至消失的解剖学特征。在同样的组培条件和相同的时间内,同一喜树叶片不同部位出现细胞壁程度不同的降解和消失现象,可能是喜树叶片因上表皮凸凹不平进而导致其不同部位与酸性培养基接触的程度不同,因而使培养基中的酸性物质对喜树叶片上表皮的不同部位影响出现异质化。本文对培养基中的酸性成分对喜树叶片细胞壁降解的影响有了进一步的认识和理解。

Influence of acid culture medium on cell wall degradation of Camptotheca acuminata leaves

During the course of plant tissue culture, Camptotheca acuminata leaf explants were observed on anatomy characteristics. We found that cell wall of C. acuminata leaves presented anatomy characteristic of weakly degrading, obviously degrading, totally degrading and cell wall disappearance under tissue culture conditions. Cell wall of identical C. acuminata leaf exhibited distinct properties in their degree of degradation and even disappearing at the same condition of tissue culture. It may be the upper epidermis protruding to influence different contacts position with culture medium and make acid material of culture medium to cause different position of C. acuminata leaf explants to appear heterogeneity.