| 枫香自然种群遗传多样性的ISSR分析 |
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| 引用本文： | 毕泉鑫,金则新,李钧敏,李建辉.枫香自然种群遗传多样性的ISSR分析[J].植物研究,2010,30(1):120-125. |
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| 作者姓名： | 毕泉鑫 金则新 李钧敏 李建辉 |
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| 作者单位： | 1.北京林业大学自然保护区学院，北京 100083；;2.台州学院生态研究所，临海 317000 |
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| 基金项目： | 浙江省自然科学基金项目(Y507660) |
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| 摘 要： | 利用ISSR分子标记技术,对浙江省内枫香自然种群的遗传多样性进行分析。用10个引物对5个枫香种群共100个个体的样品DNA进行扩增,共测得135个位点,其中多态位点为118个,多态位点百分率(P)为87.41%,Shannon指数(I)为0.4646,Nei指数(h)为0.3122,表明枫香总体水平的遗传多样性较高。各种群的多态位点百分率平均为59.11%,Shannon指数平均为0.3660,Nei指数平均为0.2543。P、I、h均显示北山种群最高,天台山种群最低。AMOVA分子差异分析显示:85.49%的变异存在于种群内,14.51%存在于种群间,基因分化系数(Gst)为0.1856,种群间的遗传分化程度较低。种群间的基因流(Nm)为2.1944。5个种群间的平均遗传距离为0.1199。利用UPGMA法对5个种群进行聚类分析,结果分为两大类群:白云山、天台山、北山和安岱后4个种群组成一大类群;大明山种群单独为另一类群。
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| 关 键 词： | 枫香 遗传多样性 遗传分化 ISSR |
Genetic Diversity in the Natural Populations of Liquidambar fornosana Revealed by ISSR Molecular Markers |
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| BI Quan-Xin, JIN Ze-Xin LI Jun-Min LI Jian-Hui.Genetic Diversity in the Natural Populations of Liquidambar fornosana Revealed by ISSR Molecular Markers[J].Bulletin of Botanical Research,2010,30(1):120-125. |
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| Authors: | BI Quan-Xin JIN Ze-Xin LI Jun-Min LI Jian-Hui |
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| Institution: | 1.College of Nature Conservation/a>;Beijing Forestry University/a>;Beijing 100083/a>;2.Ecology Institute of Taizhou University/a>;Linhai 317000 |
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| Abstract: | By using inter-simple sequence repeat(ISSR)markers technique,the genetic diversity of five populations of endangered relic shrub Liguidambar fornosana was studied in this paper.Using 10 primers,ISSR amplification was conducted in 100 individuals from 5 L.fornosana populations and 118 polymorphic loci were detected from total 135 loci.The total percentage of polymorphic loci(P)was 87.41%.The mean of Shannon's Information index(I)and Nei's gene diversity(h)at population level was 0.366 0 and 0.254 3.It sugges... |
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| Keywords: | Liguidambar fornosana genetic diversity genetic differentiation ISSR |
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