离体培养黄瓜子叶诱导雌花的研究

采用添加Spd和IAA的MS培养基培养离体黄瓜子叶，研究了Spd和IAA对雌花诱导的协同作用，及昼夜温差、培养基中N素和pH值对雌花诱导的影响。结果表明，分别添加Spd、IAA时的雌花诱导率和单株雌花数偏低或为0，12 mg·L^-1 Spd与0.01mg·L^-1 IAA 配合时的诱导效果明显高于单独处理的，而对照组未见雌花，说明Spd和IAA对雌花诱导的协同作用显著。在0、2、6、10℃昼夜温差，60、70、80、90 mmol·L^-1的N素含量和pH 5.4、5.8、6.2、6.6的培养条件下，70 mmol·L^-1 N、6℃温差和pH 6.2时的雌花诱导效果较好，表明适当提高昼夜温差、培养基中N素和pH值有利于黄瓜子叶的雌花诱导。

The Induction of Female Flowers in Cotyledons of Cucumber(Cucumis sativus L.)in Vitro

The research of the synergistic effect of Spd and IAA as well as the effects of temperature difference round the clock, N concentration and pH on the induction of female flowers in cotyledons of cucumber(Cucumis sativus L.) in vitro was carried out, in which MS basal medium supplemented with Spd and IAA was used. It was found that the induction rate and the amount of female flower in every trial were considerably low and even down to zero when Spd or IAA was respectively added to cotyledons cultured on MS basal medium, while the induction effect were obviously higher when 12 mg·L^-1 Spd and 0.01 mg·L^-1 IAA were added simultaneously, and no female flowers could be observed on the control medium, which indicated that Spd and IAA had an significant synergistic effect on the induction of female flowers. Experiment was done with N concentration of 60~90 mmol·L^-1 or with temperature difference round the clock of 0~10℃, and with pH of 5.4~6.6, the great effect on the induction of female flowers was achieved on medium of N 70 mmol·L^-1, from temperature difference 6℃ and pH 6.2, which showed that the appropriate increase of temperature difference, N concentration and pH was of benefit to the induction of female flowers in cotyledons of cucumber(Cucumis sativus L.)