| 地上部特异性启动子驱动番茄原系统素表达的载体构建及转基因植株的获得 |
| |
| 引用本文： | 赵雪芹,刘维仲,蒋红玲,张海燕.地上部特异性启动子驱动番茄原系统素表达的载体构建及转基因植株的获得[J].植物研究,2011(3):318-322. |
| |
| 作者姓名： | 赵雪芹 刘维仲 蒋红玲 张海燕 |
| |
| 作者单位： | 山西师范大学生命科学学院;中国科学院植物研究所分子发育生物学研究中心;中国科学院遗传与发育生物学研究所基因组生物学研究中心; |
| |
| 基金项目： | 国家自然科学基金委重大研究计划项目(90817010) |
| |
| 摘 要： | 克隆地上部特异表达的启动子——cab2(chlorophyll a/b binding protein 2,cab2)基因的启动子,构建该启动子驱动下的番茄原系统素(Prosystemin;PS)与GFP融合的植物表达载体并获得转基因植株。利用农杆菌介导法转化拟南芥,通过RT-PCR的方法及激光共聚焦显微镜观察启动子驱动PS-GFP的表达及其亚细胞定位。以拟南芥基因组为模板,利用高保真聚合酶获得了cab2启动子的目的片段,并将其与接GFP的番茄原系统素载体(SlPS)融合,激光共聚焦显微镜观察表明,该启动子驱动的基因正常表达和并定位于细胞质中。克隆获得到了cab2基因的启动子,该启动子能够驱动番茄原系统素和GFP的融合蛋白正常表达和定位。
|
| 关 键 词： | 拟南芥 cab2 地上部特异性启动子 系统素 |
Vector Construction of Shoot-specific Promoter Driven Prosystemin and the Acquisition of Transgenic Plants |
| |
| ZHAO Xue-Qin, LIU Wei-Zhong JIANG Hong-Ling ZHANG Hai-Yan.Vector Construction of Shoot-specific Promoter Driven Prosystemin and the Acquisition of Transgenic Plants[J].Bulletin of Botanical Research,2011(3):318-322. |
| |
| Authors: | ZHAO Xue-Qin LIU Wei-Zhong JIANG Hong-Ling ZHANG Hai-Yan |
| |
| Institution: | ZHAO Xue-Qin1,3 LIU Wei-Zhong1 JIANG Hong-Ling2 ZHANG Hai-Yan3(1.College of Life Sciences,Shanxi Normal University,Linfen 041000,2.The Center for Genome Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,3.The Research Center for Molecular and Developmental Biology,Institute of Botany,Beijing 100093) |
| |
| Abstract: | The objective of this study was to clone a shoot-specific promoter,it is the promoter of cab2(chlorophyll a/b binding protein 2,cab2) gene.The promoter can drive the expression of prosystemin(PS) and GFP fusion vector,then get the transgenic plants.The plasmids were transformed into Arabidopsis by using Agrobacterium tumefaciens-mediated method.The semi-quantitative RT-PCR method and laser scanning confocal microscope(LSCM) were used to observe the expression and subcellular localization of promoter-driven ... |
| |
| Keywords: | Arabidopsis cab2 shoot-specific promoter prosystemin |
| 本文献已被 CNKI 等数据库收录！ |
