| 拟耧斗菜昼夜差异表达ESTs的分离与分析 |
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| 引用本文： | 刘德团,李婉莎,查宏光,杨永平,胡向阳.拟耧斗菜昼夜差异表达ESTs的分离与分析[J].植物研究,2011,31(4):414-421. |
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| 作者姓名： | 刘德团 李婉莎 查宏光 杨永平 胡向阳 |
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| 作者单位： | 中国科学院昆明植物研究所;中国科学院研究生院; |
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| 基金项目： | 国家自然科学基金面上项目(30871704); 中国科学院百人计划项目 |
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| 摘 要： | 应用磁珠介导的抑制消减杂交方法，以青藏高原白马雪山流石滩地区植物拟耧斗菜为实验材料，分别在白天与夜晚采样，以白天样品为目标，晚上样品为驱动样品，分离昼夜差异表达的基因。随机挑选了96个差异ESTs克隆并进行测序，测序结果进行blastx网上数据库比对、Blast2go功能注释和KEGG代谢路径分析。结果表明：绝大部分ESTs功能与物质能量代谢、生物合成、蛋白和核酸结合、转移酶活性相关，还有少数与逆境胁迫相关等。这为进一步研究高山植物逆境适应的分子机制奠定了基础。
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| 关 键 词： | 高山植物 胁迫 抑制消减杂交 |
Isolation and Analysis of Day-night Differential Expressed ESTs from Paraquilegia microphylla |
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| LIU De-Tuan, LI Wan-Sha ZHA Hong-Guang YANG Yong-Ping HU Xiang-Yang.Isolation and Analysis of Day-night Differential Expressed ESTs from Paraquilegia microphylla[J].Bulletin of Botanical Research,2011,31(4):414-421. |
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| Authors: | LIU De-Tuan LI Wan-Sha ZHA Hong-Guang YANG Yong-Ping HU Xiang-Yang |
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| Institution: | LIU De-Tuan1,2 LI Wan-Sha1 ZHA Hong-Guang1 YANG Yong-Ping1 HU Xiang-Yang1(1.Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,2.Graduate University of Chinese Academy of Sciences,Beijing 100049) |
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| Abstract: | Suppression subtractive hybridization(SSH) based on magnetic beads technology was used to screen day-night differential expression genes of Paraquilegia microphylla living on the scree of the Baima snow mountain of Tibetan plateau.The daytime sample was used as tester and nighttime sample as driver for further analysis.Finally,96 differential ESTs between two samples were sequenced.Sequences were analyzed with BlastX,Blast2go and KEGG.The results indicated that most differential expression proteins are rela... |
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| Keywords: | alpine plant stress suppression subtractive hybridization(SSH) |
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