| 利用烟草表达人源性胰岛素样生长因子1 |
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| 引用本文： | 李源,李海权,肖刚,李建粤.利用烟草表达人源性胰岛素样生长因子1[J].植物研究,2011(3):336-340. |
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| 作者姓名： | 李源 李海权 肖刚 李建粤 |
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| 作者单位： | 上海师范大学生命与环境科学学院; |
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| 基金项目： | 上海市教育委员会重点学科建设项目资助(项目编号:J50401) |
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| 摘 要： | 构建了由花椰菜花叶病毒35S启动子引导人源性胰岛素样生长因子1基因(igf-1)的表达载体pCAM-BIA1301-35S promoter-igf-1-nos,并利用根癌农杆菌LAB4404介导,将其导入烟草。经潮霉素抗性筛选、GUS检测和PCR鉴定,获得17棵转基因植株。RT-PCR分析结果显示,igf-1能够在转基因烟草中正常转录。本试验为利用烟草以及其他双子叶植物高效表达便于分离纯化的IGF-1药用蛋白研究奠定了重要的基础。
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| 关 键 词： | 胰岛素样生长因子1基因(igf-1) 烟草 植物生物反应器 |
Producing IGF-1 in Transgenic Tobacco |
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| LI Yuan LI Hai-Quan XIAO Gang LI Jian-Yue.Producing IGF-1 in Transgenic Tobacco[J].Bulletin of Botanical Research,2011(3):336-340. |
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| Authors: | LI Yuan LI Hai-Quan XIAO Gang LI Jian-Yue |
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| Institution: | LI Yuan LI Hai-Quan XIAO Gang LI Jian-Yue(College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234) |
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| Abstract: | In this research,a new expression vector of pCAMBIA1301-35S promoter-igf-1-nos,which included the gene of hIGF-1,was constructed.The gene is promoted by CaMV 35S promoter which can be expressed in tobacco in high performance.The expression vector of IGF-1 cDNA introduced by 35S promoter was transformed into LBA4404(Agrobacterium tumefacients),and then infected tobacco through leaf-disc infection.17 transgenic tobacco plants were gained after hygromycin selection,GUS test and PCR test of DNA.It shows IGF-1 g... |
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| Keywords: | Insulin-like growth factor I(IGF-1) tobacco plant bio-reactor |
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