14个蝴蝶兰品种遗传关系的ISSR分析

采用ISSR分子标记技术对14个蝴蝶兰品种进行品种间遗传关系的研究。利用14个筛选的引物共扩增出179条带，其中多态性条带147条，多态性条带比率(PPB)为82%。品种之间的遗传相似系数范围在0.734~0.936间，说明部分蝴蝶兰品种间存在显著的遗传分化。14个引物组合可区分所有14个品种，并且检测到20条品种特异性条带，这些品种特异条带可用来鉴定供试蝴蝶兰中的10个品种。因此，ISSR分子标记能有效地进行蝴蝶兰品种鉴定。UPGMA聚类分析表明，14个品种可聚为2类，聚类情况与花色特征比较一致，但与花色的划分结果不完全相同，这可能是由于品种间杂交引起的。本文也论讨了ISSR分析结果对蝴蝶兰育种的指导意义。

Genetic Relationships Analysis of 14 Cultivars of Phalaenopsis hybrid Based on ISSR Molecular Markers

Inter-Simple Sequence Repeat(ISSR) molecular markers were used to analyze the genetic relationships of 14 Phalaenopsis cultivars with different origins and colors. Using 14 primers, we amplified 179 bands, of which 147 bands are polymorphic, and the average percentage of polymorphic bands was 82%. The range of genetic similarity was 0.734~0.936, suggesting that the substantial genetic divergence between some cultivars. ISSR markers represent a useful method for cultivar identification of Phalaenopsis, all 14 cultivars could be distinguished by these ISSR markers and 20 cultivar-specific ISSR bands were obtained for 10 of the 14 Phalaenopsis cultivars tested. Cluster analysis classified the 14 cultivars into 2 major groups. The result of UPGMA (unweighted pair-group method using arithmetic averages) clustering analysis was generally consistent with color, but there were some exceptions between clustering result and flower colors which may result from hybridization between cultivars. Article also discussed the instructional significance of experiment’s result to Phalaenopsis breeding.