钝裂银莲花花粉活力测定方法的研究

以3种花色（黄色、浅黄色和白色）钝裂银莲花为研究对象，分别采用TTC染色、I2-I染色、无机酸测定和蔗糖萌发4种方法，测定了钝裂银莲花（Anemone obtusiloba）的花粉活力，同时比较了不种方法对花粉活力的测定效果，并了解了花粉活力在一天内的动态变化情况。结果表明：（1）钝裂银莲花花粉可育率很高，达80%左右。花粉直径为28.75~33.75 m 。（2）TTC浓度影响花粉活力的测定，当浓度为0.5%时，所测花粉活力的效果较好。（3）I2-I法的染色效果不理想，染色率仅为8.33%~22.2%，且3种花色花粉活力测定数据间差异不显著。（4）3种花色钝裂银莲花花粉活力有差异,黄色最高，浅黄色次之，白色最低。TTC染色法是简单快速测定钝裂银莲花花粉活力的最适方法。一天内花粉活力在12:00~14:00最大。

Detection Methods for Pollen Viability of Anemone obtusiloba

The pollen viability of Anemone obtusiloba with three-olor flowers (yellow, pale yellow and white) was detected with four methods: TTC dyeing, I2-I dyeing, inorganic acid test, sucrose-ased germination, the determination effects of different methods were compared, and the dynamic change of pollen viability within a day was understood. The results show that: (1) The fertile rate of pollen of A.obtusiloba is high, about 80%. Pollens diameter are 28.75-33.75 m. (2) The concentration of TTC can influence the determination of pollen vitality, when the concentration was 0.5%, it was better than the others. (3) I2-I dyeing is not ideal, dyeing rate is only 8.33%-22.2%, and there is no significant difference within three-olor flowers. (4) The pollen vitality of A.obtusiloba with three-olor flowers have differences, with yellow the highest, followed by light yellow, white the minimum. TTC dyeing is a simple and rapid determination method of A.obtusiloba. Within a day, pollen vitality is maximum from 12:00-14:00 pm.