高寒藏药—抱茎獐牙菜组织培养研究及其愈伤组织有效成分的测定

从抱茎獐牙菜的胚轴、幼叶及未成熟种子诱导出愈伤组织并再生植株，试验选用ＭＳ、Ｂ５和Ｎ６三种培养基，其中以附加２．４－Ｄ３．０ｍｇ／Ｌ＋６－ＢＡ０．５ｍｇ／Ｌ的ＭＳ培养基诱导率最高；以附加６－ＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ的ＭＳ培养基分化苗频率最高；以附加２．４－Ｄ２．０ｍｇ／Ｌ＋６－ＢＡ０．５ｍｇ／Ｌ的ＭＳ培养基愈伤组织的生长最好。结果表明，外植体，培养基，激素等对愈伤组织诱导、继代和分化均有明显影响。采用高压液相色谱法（ＨＰＬＣ）测定抱茎獐牙菜愈伤组织中齐墩果酸含量的结果表明，愈伤组织中齐果墩酸含量因培养基、继代培养时间的不同而有所差异

TISSUE CULTURE AND ANTIHEPATITIS CONSTITUENT IN CALLI OF SWERTIA FRANCHETIANA H. SMITH

Calli and the regenerative plants were induced from young leaves, immature seeds and hypocotyl of Swertia franchetiana H. Smith. Three kinds of media, MS, B 5 and N 6 were set up in the experiment. Calli were established with the highest frequency onto MS medeium containing 3mg/L 2,4-D and 0.5mg/L 6BA. The regenerative plants are obtained with the highest frequency onto MS medeium, Containing 0.5mg/L 6-BA and 0.2mg/L NAA. The induction and maintenence and differentiation of the calli were affected markly by the basic media and compound 2,4-D and 6-BA in the media etc. The Oleanolic acid content in calli of Swertia franchetiana H. Smith were determined by HPLC method.