| 马铃薯杂种F1无性株系的ISSR鉴定 |
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| 引用本文： | 甘霖；于肖夏；鞠天华；于卓；蒙美莲；张自强；李长青.马铃薯杂种F1无性株系的ISSR鉴定[J].植物研究,2013,33(2):243-247. |
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| 作者姓名： | 甘霖；于肖夏；鞠天华；于卓；蒙美莲；张自强；李长青 |
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| 作者单位： | 1.内蒙古农业大学农学院，呼和浩特 010019；;2.中国农业大学农业部作物遗传改良重点实验室，北京 100193；;3.赤峰天泽生物科技有限公司，赤峰 025457 |
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| 摘 要： | 为给马铃薯新品种选育提供可靠材料，采用ISSR分子标记对2个马铃薯杂交组合‘J07-4’ב陇薯6号’和‘J07-6’ב陇薯6号’杂交种F1无性繁殖株系的真实性进行了鉴定。结果从152个ISSR引物中筛选出适于‘J07-4’ב陇薯6号’杂种F1 5个无性株系鉴定的3个ISSR引物AF18550、AW75511和AW20617及‘J07 6’ב陇薯6号’杂种F1 7个无性株系鉴定的2个ISSR引物AW20607和AF18549；以子代中含有父本特征带为主要依据，鉴定出杂种F1共12个无性繁殖株系均为真实的杂交种，并建立了杂种F1不同无性株系间的ISSR指纹图。表明ISSR分子标记技术用于马铃薯杂种F1无性株系鉴定是可行的。
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| 关 键 词： | 马铃薯 杂种F1 无性株系 ISSR鉴定 |
ISSR Identification of Clone Plants of Solanum tuberosum Hybrids F1 |
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| Institution: | 1.College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010019；2.Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture,China Agricultural University,Beijing 100193；3.Chifeng Tianze Bio Technology CO,LTD,Chifeng 025457 |
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| Abstract: | In order to provide reliable materials for potato varieties breeding, the study was to identify the authenticity of clones of hybrids F1 of two potato combinations, ‘J07-4’בLongshu No.6’ and ‘J07-6’בLongshu No.6’, using ISSR molecular markers. The results were that five primers were selected from 152 ISSR primers, primers AF18550, AW75511 and AW20617 were suitable to identify 5 clones of ‘J07-4’בLongshu No.6’ hybrids F1, and AW20607 and AF18549 were suitable to identify 7 clones of ‘J07-6’בLongshu No.6’ hybrids F1. A total of 12 clones were identified as true hybrids based on the paternal-specific bands in progeny’s bands. ISSR fingerprints of different clones of hybrids F1 were established. It showed that it was feasible to use ISSR molecular markers to identify clones of potato hybrids F1. |
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| Keywords: | Potato hybrids F1 clones ISSR identification |
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