植物叶片原生质体分离的可能机制

分析了植物叶片在分离液环境中形成原生质体的过程,文中提出,分离液配方中的酸性物质使植物叶片处于酸性环境中并导致植物正常细胞首先发生细胞壁酸性降解,随后出现原生质体脱离细胞壁进入分离液,继而又进一步发生质膜的酸性降解,使细胞核和细胞器进入分离液中,最终分离液中的细胞器以细胞核为中心进行细胞器重组,最后产生外貌形态一致的新的原生质体.植物细胞壁和质膜是植物细胞的包被系统.植物细胞包被系统的酸性降解使植物细胞器重组并产生新的原生质体成为可能.

Possible mechanism involved with isolation protoplast of leaf

The process and mechanism of leaf protoplast formation after incubation to cell wall digesting solution was investigated. The acid material in the digesting solution provided the acid environment for leaves and led to the occurrence of acid degradation of cell wall, the separation between cell wall with protoplast and the escape of protoplast from cell wall into solution. The cytoplasm membrane inside the digesting solution was degraded by the acid environment and this accounted for the escape of cell nucleus and organelles from protoplast. In digesting solution, the recombination of organelles surrounding nucle- ar and subsequent formation of new protoplast with unanimous shape took place. These results showed that plant cell wall and cytoplasm membrane is the coat system of plant cell. The acid degradation of plant cell coat system makes recombination of cell organ and production of new protoplast possible.