吴茱萸的AFLP指纹图谱的初步研究

首次报道中草药植物吴茱萸基因组DNA指纹图谱的研究。吴茱萸是贵州省内经济价值极高的中草药之一，采用扩增片段长度多态性（AFLP）技术来分析来自不同地区的石虎、疏毛、大花吴茱萸3个品种的DNA指纹图谱，从18对引物中筛选出3对引物对19份材料的DNA检测，共得到93条带，其中多态性片段57条（平均61.3%）。3对引物组合从DNA指纹图谱上将19份材料完全区分开，结果表明AFLP技术是鉴别吴茱萸相近品种的有效方法，是形态学鉴定方法的有益补充；UPGMA方法聚类分析显示19份种质材料间的相似系数为0.235～0.941,表明吴茱萸种质间的遗传多样性丰富；余庆地区种植基地的石虎和疏毛样本聚为一类，提示人工栽培影响到吴茱萸的遗传特性。

AFLP Fingerprints Analysis of Fructus Evodiae

In the present study,the DNA fingerprints of Fructus Evodiae was reported.Fructus Evodiae is one of the most commercially important herbs in Guizhou Province.Amplified fragment length polymorphism(AFLP) techniques was used to analyze the DNA fingerprints of 3 types of Fructus Evodiae including Evodia rutaecarpa var.officinalis, E. rutaecarpa var.bodinieri and E. rutaecarpa(Juss.)Benth.Three primer combinations (E-AGC/M-CAG,E-AGC/M-CTG and E-ACG/M-CTG) screened out from eighteen primer pairs were used to conduct genetic analysis and variety identification of nineteen materials of Fructus Evodiae.93 bands were generated and 57 fragments appeared to be polymorphic(61.3% on average).The fragment profiles of the nineteen materials were obtained and could be distinguished from each other. The results showed that AFLP was an efficient method to identify the germplasms of Fructus Evodiae and could be a supplement for the morphological identifcation methods for Fructus Evodiae.UPGMA cluster analysis was also performed.Genetic similarity coefficient(SC) ranged from 0.235 to 0.941,indicating that Fructus Evodiae is rich in genetic diversity. Dendrogram revealed that E. rutaecarpa var.officinalis and E. rutaecarpa var.bodinieri from Yuqing region were clustered into one group,which suggested that human cultivation activities have influenced on the genetic characteristics of Fructus Evodiae.