南高丛蓝莓快繁技术体系研究简

以南高丛蓝莓试管无菌丛生芽为材料，对南高丛蓝莓丛生芽的诱导与增殖、继代次数对丛生芽诱导增殖的影响、瓶内生根、瓶外生根、不同生根方式试管苗移栽成活率的大小进行了研究。南高丛蓝莓丛生芽诱导与增殖培养基以WPM+ZT 2.0 mg·L^-1较佳，增殖倍数可达3.50；继代6次丛生芽增殖倍数可达24.00；瓶内生根生根培养基以WPM+ZT 0.5 mg·L^-1+IBA 0.1 mg·L^-1为佳，生根率可达80.73%±3.17%，生根周期为100 d；试管芽用25 mg·L^-1 IBA溶液浸蘸10 s，以1/6 WPM为营养液加珍珠岩作基质，生根率可达到80.00%±5.00%，生根周期为40 d；瓶外生根试管苗移栽成活率是瓶内生根试管苗的2倍。基本建立了南高丛蓝莓的试管快繁技术体系，为南高丛蓝莓的工业化育苗奠定了技术基础。

Rapid Propagation Technique System of Blueberry

Budding stems of Vaccinium corymbosum hybrid were used as the test materials to systematically investigate tissue culture. Proliferation and induction of cluster buds, effects on cluster buds induction and proliferation of the subculture time, test-tube rooting, ex vitro rooting, transplantation survival rate with these two different rootings in V.corymbosum hybrid were researched with tissue culture. The optimal proliferated and induced medium for V.corymbosum hybrid was WPM medium with 2.0 mg·L^-1 ZT, and proliferation rate can reached 3.5; the proliferation rate can reached 24.00 with six times of subculture culture. The optimal medium for test-tube rooting was WPM medium with 0.5 mg·L^-1 ZT, 0.1 mg·L^-1 IBA with rooting rates of 80.73%±3.17%, and the rooting culture time is 100 d. The best methods for ex vitro rooting was taking explants diping in solution with 25 mg·L^-1 IBA for 10s, and the nutrient solution is 1/6 WPM with the perlite as support materials with rooting rates of 80.00%±5.00%, and the rooting culture time is 40 d. The survival rate of ex vitro rooting is two times to test-tube rooting. The rapid propagation technique system have established and laid the technical foundation on industrialized seedling.