| 自然干燥紫萼藓总RNA提取方法 |
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| 引用本文： | 沙伟,闫苗苗,吕凤香.自然干燥紫萼藓总RNA提取方法[J].植物研究,2006,26(6):715-717. |
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| 作者姓名： | 沙伟 闫苗苗 吕凤香 |
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| 作者单位： | 齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006 |
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| 基金项目： | 国家自然科学基金;黑龙江省教育厅海外学人合作项目 |
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| 摘 要： | 介绍一种适合富含酚类、萜类等次生物质的干燥紫萼藓的总RNA的提取方法—SDS/酸酚法。采用SDS做为去污剂，用水饱和酚、氯仿和异戊醇进行抽提以去除蛋白、酚类等次生物质，醋酸钾和无水乙醇去除多糖等物质，最后LiCl沉淀获得总RNA。该方法不但获得了完整性好和纯度高的RNA，而且操作简单，成本也较低，对其他富含酚类、萜类等次生物质的干燥植物组织的总RNA的提取具有借鉴意义。
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| 关 键 词： | 紫萼藓 RNA提取 SDS |
| 收稿时间： | 2005-04-22 |
| 修稿时间： | 2005年4月22日 |
Extraction of totle RNA from dry Grimmia pilifera |
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| SHA Wei,YAN Miao-Miao,L Feng-Xiang.Extraction of totle RNA from dry Grimmia pilifera[J].Bulletin of Botanical Research,2006,26(6):715-717. |
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| Authors: | SHA Wei YAN Miao-Miao L Feng-Xiang |
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| Institution: | Department of Biology, College of Life Science and Engineering, Qiqihar University, Qiqihar 161006 |
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| Abstract: | An improved SDS/acid and phenol method was presented here to be suitable for isolation of totle RNA from dry Grimmia pilifera, a plant contained abundant secondary metaboliteso Using chemical SDS as detergent in the extraction buffer, LiCl precipitation of RNA was performed finally after removing of proteins, hydroxybenzene et al. by subsequent H2O--saturated phenol, chloroform and eyewinker. The method yielded total RNA not with integrality and high purity but with simple manipulation and cheapness. It may be applied to other dry plants full of secondary metabolites. |
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| Keywords: | Grimmia pilifera RNA extraction SDS |
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