环磷酰胺诱发蚕豆体细胞遗传损伤的研究

利用蚕豆根尖研究环磷酰胺的遗传毒性效应,结果表明:环磷酰胺(0.1～5.0 mg/mL)能够降低蚕豆根尖细胞有丝分裂指数,使根尖细胞中具有微核、核出芽及核固缩的细胞明显增多,并诱发染色体结构和行为异常,产生染色体断片、滞后和桥.环磷酰胺处理组根尖中具有核固缩和微核的细胞数呈剂量依赖性增加,且与作用时间呈正相关,而分裂指数的降低也具有剂量和时间效应关系.研究结果表明,低浓度长时间接触或高浓度短时间接触环磷酰胺均可产生遗传毒害,因此,有关的作业人员应注意防护.

Genotoxicity of cyclophsphamide on root tip cells of broad bean (Vicia faba)

The genotoxic effects of cyclophsphamide (CP) in broad bean (Vicia faba) root tips were investigated. The results indicated that CP caused the mitotic index decrease,while the frequencies of abnormal cells, such as chromosomal aberrations (CA) including chromosomal segments, bridges and laggards, micronucleus (MN), nuclear buds and pycnosis, increased significantly in CP exposed roots. The number of cells with MN and pycnosis increased with the concentration of the treatments and the length of exposure periods. But the mitotic index had a negative response to the treated concentration and duration time. Our results indicated that the exposure to CP at lower concentrations for long duration can cause the same cytogenetic damage as at high concentrations for short exposure, so we should pay more attention to the occupational exposures.