沙枣组织脱分化培养与快繁体系建立的研究

研究了以沙枣的种子为外植体诱导形成愈伤组织以及植株再生的过程，对培养中出现的愈伤组织和不定芽的类型进行了探讨。通过正交试验及单因素试验方法，确定了沙枣快繁体系的最适培养方案：①诱导材料：苗期为10 d的沙枣无菌苗子叶；②有效愈伤组织诱导培养基：MS+NAA 0.5 mg·L^-1+BA 2.0 mg·L^-1+3%蔗糖；③有效芽的分化培养基：MS+NAA 0.1 mg·L^-1+BA1.0 mg·L^-1+3% 蔗糖；④壮苗培养基：MS+NAA 0.1mg·L^-1+BA 1.0 mg·L^-1+GA 30.2 mg·L^-1+3%蔗糖；⑤生根培养基：1/2MS+ABT生根粉（1号）1.0 mg·L^-1+1.5%蔗糖。

Induction differentiation and plant regeneration of Elaeagnus angustifolia

Elaeagnus angustifolia L. belonging to Elaeagnaceae is a very important tree species, mainly distributed over the northwest of China and has ability of resistance to saline-alkali. In order to accelerate reproduction and keep its excellent ability, the formation of cotyledon callus and plantlet regeneration from seed explants of Elaeagnus angustifoli were studied. And the organogenesis types of callus and regeneration shoots were discussed. The optimal culture conditions were established with the methods of the orthogonal tests and single factor tests. They are as follows:①explants: the cotyledon of 10 days old seedling;②Efficiency differentiation induction media: MS+0.5 mg·L^-1 NAA+2.0 mg·L^-1 BA+3%sucrose;③Efficiency seedling induction media: MS+0.1 mg·L^-1 NAA+1.0 mg·L^-1 BA+3%sucrose;④Robust seedling media: MS+0.1 mg·L^-1 NAA+1.0 mg·L^-1 BA+0.2 mg·L^-1 GA3+3%sucrose;⑤Rooting media: 1/2MS+1.0 mg·L^-1 ABT1.0 mg·L^-1 +1.5% sucrose.