| 红松RAPD实验中各组成成份含量对实验结果的影响 |
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| 引用本文： | 张恒庆,安利佳.红松RAPD实验中各组成成份含量对实验结果的影响[J].植物研究,1999,19(2):183-188. |
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| 作者姓名： | 张恒庆 安利佳 |
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| 作者单位： | 东北林业大学森林植物生态学开放研究实验室，大连理工大学化工学院 |
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| 基金项目： | 黑龙江省杰出青年科学基金 |
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| 摘 要： | 本文采用Ｐｒｏｍｅｇａ的ＰＣＲ反应系统，以从红松幼叶中提取的总ＤＮＡ为模板，进行随机扩增多态ＤＮＡ（ＲＡＰＤ）实验。
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| 关 键 词： | 红松 成分 随机扩增 多态DNA PCR |
EFFECT ON THE EXPERIMENTAL RESULTS OF THE CONTENT OF COMPOSITIONS IN RAPD EXPERIMENT OF PINUS KORAIENSIS |
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| Zhang Heng-qing,An Li-jia,Zu Yuan-gang.EFFECT ON THE EXPERIMENTAL RESULTS OF THE CONTENT OF COMPOSITIONS IN RAPD EXPERIMENT OF PINUS KORAIENSIS[J].Bulletin of Botanical Research,1999,19(2):183-188. |
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| Authors: | Zhang Heng-qing An Li-jia Zu Yuan-gang |
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| Institution: | Zhang Heng-qing 1 An Li-jia 2 Zu Yuan-gang 1 |
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| Abstract: | The PCR reaction system by Promega was employed. The DNA templates of Pinus koraiensis extracted from young needles were amplified in RAPD. The effect of content of Mg 2+ , dNTP, DNA templates, primers and DNA polymorase on experimental results were tested and the optimal reaction system of RAPD for korean pine was determined. |
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| Keywords: | Pinus koraiensis RAPD Composition |
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