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| 对TMV不同抗性番茄品种的叶绿体DNA限制性内切酶酶谱分析 | |
| 引用本文： | 黄永芬,汪清胤,孙德君,傅桂荣,王海廷,邢怡,程振起.对TMV不同抗性番茄品种的叶绿体DNA限制性内切酶酶谱分析[J].植物研究,1995,15(3):391-396. |
| 作者姓名： | 黄永芬 汪清胤 孙德君 傅桂荣 王海廷 邢怡 程振起 |
| 作者单位： | 1. 哈尔滨师范大学生物系, 哈尔滨 150080; 2. 治华大学生命科学与技术系, 北京 100084 |
| 摘 要： | 选用对TMV有抗性和敏感的番茄品种、制备其ct-DNA, 用限制性内切酶BumHI、EcoRI和PstI完全酶解, 三种酶切图谱与前人报道一致, 由酶切片段计算番茄ct-DNA。分子量约为156.9kb。比较抗性和敏感品种的ct-DNA图谱, 发现三种酶切图谱均存在差异, 但由差异片段计算分子量之和又很除近。我们推测这是由于检基顺序变异或小段DNA顺序插入或缺失所造成, 由此证明, 叶绿体基因组与核中的TMV抗性基因, 共同决定着植物体对TMV的抗性。 |
| 关 键 词： | 番茄 TMVct-DNA 限制性内切酶 |
| 收稿时间： | 1995-02-01 |
ANALYSIS OF RESTRICTION ENZYMES OF CHLOROPLAST DNA IN TOMATO VARIETIES WHICH HAVE DIFFEERENT RESISTANCE TO TOBBACCO MOSSIC VIRUS |
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| Huang Yong-fen, Wang Qing yin, Sun De jun Fu Gui rong,Wang Hat ting,Xing Yi,Cheng Zhen-qi.ANALYSIS OF RESTRICTION ENZYMES OF CHLOROPLAST DNA IN TOMATO VARIETIES WHICH HAVE DIFFEERENT RESISTANCE TO TOBBACCO MOSSIC VIRUS[J].Bulletin of Botanical Research,1995,15(3):391-396. | |
| Authors: | Huang Yong-fen Wang Qing yin Sun De jun Fu Gui rong Wang Hat ting Xing Yi Cheng Zhen-qi |
| Institution: | 1. Department of Biology, Harbin Normal University, Harbin, 150080; 2. Department of Biological Science and Technology, Tsinghua University, Beijing 100084 |
| Abstract: | We analyze ct-DNA of tomato which is resistant and sensitive to TMV with restriction enzyme. Our electrophoretogram of BamHI, EcoRI. Pst I is similar to other report.Molecular weight of ct-DNA is 156. 9kb, it is similar to others' study.We find some difference in BamHI EcoRI, PSt I electrophoretogram between resistant group and sensitive group. We guess this maybe the alternation of base sequence or insertion or deletion of short DNA sequence. We also infer that nucleus genome and chloroplast genome together decide the resistance of plant to Tobbacco Mossic Virus. |
| Keywords: | Tomato TMV ct-DNA Restriction endonucleases |
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