狭叶黄芩组织培养再生体系的建立

以狭叶黄芩的茎段为外植体，研究不同消毒剂处理、不同植物生长激素配比对狭叶黄芩茎段腋芽诱导、愈伤组织诱导、丛生芽分化、增殖、生根及移栽的影响。结果表明：最佳消毒方式为0.1% HgCl₂消毒5 min，污染率最低为8.25%；诱导腋芽最佳培养基为MS+1 mg·L^-1 6-BA+1 mg·L^-1 NAA，诱导率可达73.66%；诱导愈伤最佳培养基为MS+1 mg·L^-1 6-BA+1 mg·L^-1 2-4D，诱导率为91.33%；愈伤组织分化的最佳培养基为MS+1 mg·L^-1 6-BA+0.5 mg·L^-1 NAA，分化率为44.71%。芽增殖的最佳培养基为MS+1 mg·L^-1 6-BA+0.5 mg·L^-1 NAA，其芽增殖倍数为5.85；最佳生根培养基为1/2MS+0.2 mg·L^-1 IBA，生根率可达到74.07%；试管苗移栽时蛭石：珍珠岩：园土比例按1：1：3的体积比搭配使用，移栽成活率最高达到79.24%，并且植株生长旺盛。本研究建立狭叶黄芩再生体系，为狭叶黄芩野生资源在妥善的保护基础上开发应用提供一定的理论支持。

Establishing Tissue Culture and Regeneration System of Scutellaria regeliana

We studied the effects of different disinfectant treatments and different plant growth hormone ratios on axillary bud induction, callus induction, cluster bud differentiation, proliferation, rooting and transplantation of Scutellaria regeliana stem segments with the stem segments of S.regeliana as explants. The best disinfection method was 0.1% HgCl₂ for 5 min, with the lowest pollution rate of 8.25%; the best medium for inducing axillary buds was MS+1 mg·L^-1 6-BA+1 mg·L^-1 NAA, with the induction rate of 73.66%; the best medium for inducing callus was MS+1 mg·L^-1 6-BA+1 mg·L^-1 2-4D, with the induction rate of 91.33%; and the best medium for inducing callus differentiation was MS+1 mg·L^-1 6-BA+0.5 mg·L^-1 NAA, with the differentiation rate of 44.71%. The optimum medium for bud multiplication was MS+1 mg·L^-1 6-BA+0.5 mg·L^-1 NAA, and the multiple of bud multiplication was 5.85; the optimum medium for rooting was 1/2MS+0.2 mg·L^-1 IBA, and the rooting rate could reach 74.07%; the volume ratio of vermiculite:perlite:pearlite:garden soil was 1:1:3, the highest survival rate of transplantation was 79.24%, and the plant grew vigorously. In this study, the regeneration system of S.regeliana was established to provide theoretical support in the development and application of S.regeliana wild resources on the basis of proper protection.