22种木莲属植物亲缘关系的ISSR分析

利用ISSR标记分析技术探讨了22种木莲属植物的遗传亲缘关系。选用10对ISSR引物组合对木莲属22个种的基因组进行分析。结果表明：22个种共扩增出595条DNA带，其中多态性条带占94.45%；根据Nei Li遗传相似性系数在0.68处，UPGMA聚类结果将22个种划分为6个类群，在0.70处进一步可分为12个亚类群；22种木莲属植物的Nei-Li遗传相似性系数变化范围为0.605(乳源木莲与球果木莲之间)～0.956(中缅木莲与滇南木莲之间)，平均遗传相似性系数为0.698。说明木莲属植物种间差异性大。其中中缅木莲、滇南木莲之间遗传相似性系数为0.956，两者亲缘关系最近。该结果同时支持将巴东木莲、乳源木莲、滇桂木莲分别作为独立的种。

Analyses of the Genetic Relationships Among 22 Species of Manglietia Plants Using ISSR Markers

The genetic relationships among 22 species of Manglietia plants were analyzed by using inter-simple sequence repeat（ISSR) molecular-marked technique. Ten ISSR primers were selected to assess the genomes of 22 species of Manglietia plants. The result showed that a total of 595 DNA bands were amplified and 562 of which (94.45%) were polymorphic. According to the Nei-Li genetic similarity of 0.68, UPGMA method cluster analysis indicated that these 22 species were classified into 6 cluster groups, and they were classified into 12 sub-cluster groups with the genetic similarity of 0.70. The Nei-Li genetic similarities of 22 species of Manglietia ranged from 0.605 (between M.yuyuanensis and M.conifera) to 0.956 (between M.hookeri and M.wangii). The average genetic similarity is 0.698, which suggests that Manglietia have a plenty of inter-species diversity. The genetic similarity coefficient between M.hookeri and M.wangii is 0.956, and their genetic relationship is actually the closest. The result supports the classification of M.patungensis, M.yuyuanensis and M.forrestii as independent species respectively.