几种植物生长调节物质对大花蕙兰组培原球茎增殖的影响

采用KC 培养基, 分别添加不同浓度的BA、KT、NAA 及KT+NAA 组合, 对大花蕙兰初代培养已分化出的原球茎进行了继代培养。结果发现:①BA 可加快原球茎的增殖速度, 但浓度超过0.5 mg/L 后增殖速度有所下降, 且原球茎变小呈暗绿色。②KT 的促进增殖效果好于BA, 但浓度超过1.0 mg/L 时, 增殖速度也有所下降, 原球茎变小。③NAA 促进原球茎增殖的效果明显好于BA、和KT, 但浓度大于1.0 mg/L 后增殖不再加快, 而且部分原球茎有变褐现象。④在KT 0.5~0.7 mg+NAA 0.7~1.0 mg/L 范围组合的KC 培养基上, 大花蕙兰原球茎增殖的速度和质量是比较理想的。考虑到较低的细胞分裂素和植物生长素有利于植物增殖过程中遗传性的稳定, 选择KT0.5 mg+NAA 0.7 mg/L 的KC 培养基作为大花蕙兰原球茎增殖培养基。

Influence of several plant growth substance on propagation of GGB of Cymbidium grandiflorum

By using KT medium which to be added different content of plant growth substance such as BA, KT, NAA and the combination of KT and NAA, we grant on the subculture of active green globlar body (GGB)of Cymbidium grandiflorum for stand and got these results:①BA can speed the propagation of GGB, but when the content was more than 0.5 mg/L, the propagation speed got down and the green globlar body became smaller and black green.②The propagation effect of KT was better than BA, but when the content of KT was more than 1.0 mg/L, the propagation speed got down, and GGB became smaller.③NAA was benefit to the propagation of GGB, the effect was obviously better than BA and KT, but when the content was more than 1.0 mg/L, the propagation speed didn't change, and GGB became brown partly.④under the combination of KT 0.5~0.7 mg/L and NAA 0.7~1.0 mg/L., the propagation speed and quality of GGB were ideal.Because the less content of plant growth substance have an advantage of the heredity stability.We choose the combination of KT 0.5 mg+NAA 0.7 mg/L.as the propagation medium of GGB.