| 几丁质酶基因表达载体构建及转化烟草的研究 |
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| 引用本文： | 徐香玲,李集临,马兴红.几丁质酶基因表达载体构建及转化烟草的研究[J].植物研究,2002,22(3):T002-T003. |
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| 作者姓名： | 徐香玲 李集临 马兴红 |
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| 作者单位： | 1. 哈尔滨师范大学生物系,哈尔滨,150080
2. 东北农业大学,哈尔滨,150030 |
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| 摘 要： | 构建了一个含有多个调控序列的带有几丁质酶基因的植物表达载体，通过发根农杆菌的Ri质粒介导转化烟草的三个品种：K326，RG11，VA116，在卡那霉素抗性培养基上筛选，获得了7株再生植株，以PCR检测、DNA斑点杂交证明表达载体构建成功，几丁质酶基因导入烟草并整合到烟草基因组中。
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| 关 键 词： | 几丁质酶 基因表达载体 构建 转化烟草 Ri质粒 |
RESEARCH ON CONSTRUCTION OF AN EXPRESSION VECTOR OF CHITINASE GENE AND ITS TRANSFORMATION INTO TOBACCO |
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| XU Xiang ling,LI Ji lin,MA Xing hong.RESEARCH ON CONSTRUCTION OF AN EXPRESSION VECTOR OF CHITINASE GENE AND ITS TRANSFORMATION INTO TOBACCO[J].Bulletin of Botanical Research,2002,22(3):T002-T003. |
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| Authors: | XU Xiang ling LI Ji lin MA Xing hong |
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| Institution: | XU Xiang ling 1 LI Ji lin 1 MA Xing hong 2 |
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| Abstract: | Expression vector containing a chitinase gene and some regulatory sequences was in this reseach.Using the Ri plasmid of Agobacterium.rhizogenes,the chitinase gene was introduced into tobacco,three cultivars of tobacco.Seven plants were proved of be transformed tobacco by PCR detection,by dot blot hybridization.The result sussgested that the construction of the expression vector is successful and the chitinase gene had integrated into tobacco genome. |
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| Keywords: | expression vector chitinase gene tobacco Ri plasmid |
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