刺五加和短梗五加叶片初生代谢的发育特异性调控研究

利用GC-MS技术对刺五加（Acanthopanax senticosus（Rupr. et Maxim.） Harms）和短梗五加（Acanthopanax sessiliflorus（Rupr. et Maxim.） Seem）叶片初级代谢产物进行了代谢组学分析，运用PCA和OPLS-DA方法分析后，将刺五加和短梗五加叶片划分为3个发育时期：生长期（Growth period）、旺盛期（Exuberant period）、凋落期（Autumn period）。刺五加叶片3个时期筛选出糖类、氨基酸、有机酸、脂肪酸、多元醇等共53个差异化合物，短梗五加叶片3个时期筛选出糖类、氨基酸、有机酸、脂肪酸、多元醇等共51个差异化合物。进一步分析表明，刺五加和短梗五加叶片在旺盛期和凋落期之间差异化合物最多，其中刺五加主要是糖类、有机酸；短梗五加则除了上述两类化合物外，还包括脂肪酸、多胺类化合物。两者作为同属植物，在差异化合物的组成类型方面具有较高的相似性。结果初步揭示了2种同属药用植物叶片不同时期初级代谢物的积累模式，为2种植物叶片利用提供理论基础。

Development-specific Investigation of Leaf Primary Metabolism of Acanthopanax senticosus and Acanthopanax sessiliflorus

Metabolomics analysis of Acanthopanax senticosus(Rupr.et Maxim.)Harms and Acanthopanax sessiliflorus(Rupr.et Maxim.)Seem leaves was performed using GC-MS technology.With analysis of PCA and OPLS-DA,the leaves of A.senticosus and A.sessiliflorus were divided into three developmental periods:growth period,exuberant period,and autumn period.A total of 53 differential compounds such as sugars,amino acids,organic acids,fatty acids and polyols were filtered from the leaves of A.senticosus at three periods.A total of 51 differential compounds such as sugars,amino acids,organic acids,fatty acids and polyols were filtered from the leaves of A.sessiliflorus at three periods.Further analysis showed that the leaves of A.senticosus and A.sessiliflorus had the most differential compounds in the exuberant period and autumn period.Among these differential compounds,the leaves of A.senticosus mainly included sugars and organic acids.The leaves of A.sessiliflorus also included fatty acids and polyamine compounds except for sugars and organic acids.The results initially reveal the accumulation patterns of primary metabolites in the two medicinal plants from the same genus at different periods,and provid a theoretical basis for the leaf utilization of the two plants.